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KMID : 0829320070100010049
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Korean Journal of Clinical Microbiology
2007 Volume.10 No. 1 p.49 ~ p.53
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Evaluation of a Colorimetric Broth Microdilution Method for Antimicrobial Susceptibility Testing Using 2,3,5-Triphenyltetrazolium Chloride
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Lee Dae-Dong
Lee Eun-Yup
Jeong Seok-Hoon
Chang Chul-Hun L.
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Abstract
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Background: The broth microdilution susceptibility testing method is considered a standard for determining minimum inhibitory concentrations, and the addition of the redox indicator 2,3,5-triphenyltetrazolium chloride (TTC) to the broth microdilution method simplifies and increases its objectivity. The current study evaluated the usefulness of a TTC-modified broth microdilution method for antimicrobial susceptibility test of frequently encountered clinical isolates.
Methods: The minimal inhibitory concentrations (MICs) of 10 antimicrobials for 111 clinical isolates of four bacterial species, Staphylococcus aureus, Escherichia coli, Enterobacter cloacae, and Acinetobacter baumannii, were investigated by a modification of the Clinical and Laboratory Standards Institute (CLSI)-recommended broth microdilution method with the addition of 2,3,5-triphenyltetrazolium chloride (TTC). The inhibitory effects of TTC against 192 strains of 22 bacterial species isolated from clinical specimens were also evaluated.
Results: The number of colonies of all 192 strains of 22 bacterial species grown on TTC-containing Mueller-Hinton agar did not differ from those grown on Mueller-Hinton agar only. The MICs with TTC were within 2 dilutions of those obtained by the CLSI method in 569 (97.6%) of 583 organism-antimicrobial agent combinations.
Conclusions: The colorimetric MIC method using TTC may be a useful surrogate of antimicrobial susceptibility
testing for most of the frequently isolated bacteria. (Korean J Clin Microbiol 2007;10:49-53)
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KEYWORD
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Susceptibility tests, Colorimetric assay, 2, 3, 5-triphenyltetrazolium chloride, Escherichia coli, Staphylococcus aureus, Enterobacter cloacae, Acinetobacter baumannii
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